Lionel Messi's fired in a spectacular solo goal to put the visitors ahead shortly before Gerard Piqué doubled their lead with a tap-in.
Barca crushed PSV back in September during the first matchday, and Lionel Messi got a hat trick in that one.
PSV unlucky not to go into the break at least one goal in front after twice hitting the frame of the goal.
Jose Mourinho threatens to drop Manchester United players who show 'no heart'
Victor Lindelof faces a number of weeks on the sidelines with what appeared to be a groin injury sustained at the weekend. You probably thought Jose told them to start slow. "I want them to start strong and have a go nearly immediately".
Atletico Madrid extended their advantage at the top of Group A with a 2-0 win over already-eliminated Monaco.
He overtakes long-time rival Ronaldo who bagged 105 UCL goals for Real Madrid between 2009 to 2018, before leaving for Juventus in the summer.
Barcelona had a lot of the ball in the first 15 minutes, but it was PSV that were having the better moments in the final third, with Hirving Lozano and Steven Bergwijn both causing problems in wide positions.
De Jong grabbed a consolation goal for the home fans with an 83rd-minute header to set up a frenetic late assault, but despite pouring forward in the closing minutes PSV could not find an equalizer.
Pakistan not honest in bringing 26/11 perpetrators to justice, says India
Yet, the masterminds behind the 26/11 attacks, including Jamaat-ud-Dawa chief Hafiz Saeed, continue to roam freely in Pakistan. Hafiz Saeed was put under house arrest after the 26/11 Mumbai terror attack, but was freed by a court in Pakistan in 2009.
Here's how to watch PSV versus Barcelona online. Dembele scored a goal as Barcelona beat PSV at Camp Nou in their first Group B match this season, but could not add to his tally in Eindhoven.
The No.10 has not featured in the middle of the attack since the start of the Luis Enrique era, before he switched places with Suarez. This goal is not even a surprise to see anymore from the Argentine but we should not play down just how hard and spectacular this goal is.
It led to a nervy end to the match for Barca, and Denzel Dumfries had a strong injury-time penalty shout turned down after Clement Lenglet appeared to catch him in the box with a clumsy challenge.
Zoet had to keep out a powerful Messi strike early in the second period as Barcelona started on the front foot.
US service members killed in Afghanistan roadside bombing
Earlier this month, Afghan President Ashraf Ghani said more than 28,000 Afghan police and soldiers had been killed since 2015. Special Operations troops often partnering with Afghan commandos to target the Islamic State and, occasionally, al-Qaeda.